Rapid and reliable purification qiagen provides a variety of qiaamp genomic dna kits for fast, efficient and easy dna purification figures 1 and 2. The qiaamp fast dna tissue kit allows rapid purification of dna from solid. Qiaamp dna mini kit and qiaamp dna blood mini kit 022003 3 contents kit contents 5 storage 6 equipment and reagents to be supplied by user 6 product use limitations 7 product warranty and satisfaction guarantee 7 technical assistance 7 safety information 8 introduction 9 purification of viral rna and dna 9 purification of dna. Purification of total dna from animal blood or cells dneasy 96 protocol.
Plasmid or cosmid dna purification using the endofree plasmid maxi kit 36 plasmid or cosmid dna purification using. Tianquick ffpe dna kit dp330 dna extraction kit for fooddp32. Qiaquick dna cleanup guide qiaquick kits can be used to cleanup the following enzymatic reactions. Standard operating procedure sop for dna extraction with qiaamp mini kit i. Although the ctab method and commercially available kits gave high quality dna. Qiagen, hilden, germany have been used for the extraction of dna. Manual protocols using either a microcentrifuge or a vacuum manifold.
Dnaquick plant systemdp321 tianamp n96 blood dna kit dp314 tianamp dna rna isolation kit dp. The kit uses qiagen s secondgeneration inhibitor removal technology irt, and is intended for use with environmental samples containing high humic acid content, including difficult soil types such. Anionexchangebased qiagen tips yield transfection grade dna. Purification of total dna from animal tissues spincolumn protocol 32 protocol. Qiaex ii agarose gel extraction protocol this protocol is designed for the extraction of 40bp to 50kb dna fragments from 0. Isolate a suitable piece of tissue and place in a uvcrosslinked 1. Qiagen dna extraction kits for sample preparation for. Omnigeneoral om505 microbial dna and rna purification protocol using qiagen qiaamp minelute virus spin kit this laboratory protocol is used for the preparation of saliva samples collected and stabilized in omnigeneoral for subsequent extraction of microbial nucleic acids using the qiagen qiaamp minelute virus spin kit. A simplified universal genomic dna extraction protocol suitable for pcr. Qiaamp genomic dna kits enable purification of genomic, mitochondrial, viral, bacterial and. Up to 400 mg agarose can be processed per spin column. Qiaquick gel extraction kit protocol using a microcentrifuge this protocol is designed to extract and purify dna of 70 bp to 10 kb from standard or lowmelt agarose gels in tae or tbe buffer. Tianamp virus dnarna kit dp315 magnetic genomic dna kit dp329 tianamp stool dna.
Omnigeneoral om505 microbial dna and rna purification. Dna purification using the qiaamp dna mini kit or the. Australia qiagen pty ltd po box 25 clifton hill victoria 3068 abn 75 072 382 944 orders 0394893666 fax 0394893888 technical 0394893666 canada qiagen. From solutions from gels qiaquick qiaquick pcr nucleotide qiaquick gel qiaquick gel purification kit removal kit extraction kit extraction kit. In this stepbystep dneasy visual protocol, youll see how to extract genomic dna from various samples in just 20 minutes. Qiaamp dna mini kit and qiaamp dna blood mini kit 022003 3 contents kit contents 5 storage 6 equipment and reagents to be supplied by user 6 product use limitations 7 product warranty and satisfaction guarantee 7 technical assistance 7 safety information 8 introduction 9 purification of viral rna and dna 9 purification of dna from urine 9.
Contamination of qiagen dna extraction kits with legionella dna. Viral rnadna purification thermo fisher scientific us. The kingfisher system technology provides an efficient workflow solution. Standard operating procedure sop for dna extraction with. Under the hood, add 1 ml of xylene, using steril tips, to each sample. The qiaamp dna blood mini kit provides silicamembranebased dna purification. Qiagen dna extraction kits for sample preparation for legionella pcr are not suitable for diagnostic purposes. Automated purification and extraction thermo fisher. A simple and efficient genomic dna extraction protocol for large scale genetic analyses of plant biological systems. The dneasy powersoil pro kit comprises a novel and proprietary method for isolating microbial genomic dna from environmental samples. The kit uses qiaamp minelute spin columns for purification of highquality dna with flexible elution volumes.
The ht viral total nucleic acid extraction chemistry is designed for highthroughput, automated extraction of viral rna and dna. Formulas for qiagen kit buffers genesee scientific. A simple and efficient genomic dna extraction protocol for. Transfer the flowthrough fraction from step 5 into a new tube not supplied without disturbing the celldebris pellet. Qiagen plasmid purification handbook harvard university.
Qiaquick pcr purification kit protocol using a microcentrifuge this protocol is designed to purify single or doublestranded dna fragments from pcr and other enzymatic reactions see page 8. Scope and purpose qiagen qiaamp kits provide a fast, easy, and reliable method to purify total dna from small quantities of human tissue or blood. In the tilburg laboratory, the qiagen columns qiaamp dna mini kit. It has not been thoroughly tested and optimized by qiagen. Qiagen kits and silicamembrane kits are actually the extra steps after. Qiaamp dna mini kit and qiaamp dna blood mini kit handbook. Qiagen allprep dna rna ffpe kit protocol pugh lab printed. Pdf qiagen dna extraction kits for sample preparation for. Qiaex ii handbook qiaex ii agarose gel extraction protocol. This protocol is designed for purification of total dna from grampositive bacteria.
Pdf contamination of qiagen dna extraction kits with. The qiaamp dna blood mini kit is designed for processing up to 200. Forceps are to be sterilized in fine science tools heat block at. The maxwell ht viral tna kit extracts viral rna and viral dna from samples such as transport media, seurm, plasma and other viral test samples. Dna can be purified using many different methods and the downstream application determines how pure the dna should be.
Maxwell ht viral tna kit automated viral rna extraction. Qiagen genomic tip kit algimed company, llc belarus. Tianamp micro dna kit dp316 tianamp feedstuff animal dna kit. The qiaamp dna micro kit uses wellestablished technology for purification of genomic. Recovery of dna from the columns is highly efficient, and pcr inhibitors are effectively reduced.
Qiaamp mini spin columns can be easily processed in a centrifuge or on vacuum manifolds. Qiagen distributors please see the last page for contact information for your local qiagen distributor. Determination of concentration, yield, purity, and length of dna. These are available online in convenient and compact pdf. Qiagen plasmid purification handbook 082003 9 product use limitations qiagen, qiafilter, and endofree plasmid kits are developed, designed and sold for.
Both allow purification of dna of up to 150 kb in size. The qiaamp dna investigator kit provides purification of genomic dna from a wide range of forensic and human identity samples, such as casework samples, including dried blood, bone, and sexual assault samples, swabs, and filters. All of our viral spincolumn kits including purelink viral rna dna mini kit, thermo scientific genejet viral dna rna purification kit and purelink pro 96 viral rna dna kit with 96well plate are designed for fast and easy isolation of viral rna or dna. The protocol describes the preliminary harvesting of bacteria and incubation with lysozyme to lyse their cell walls before dna purification. Solutions that contain ethanol, isopropanol or mops should be sterilized by filtration only. Comparison of six genomic dna extraction methods for molecular. The kit uses qiagen s secondgeneration inhibitor removal.
Purify highquality, genomic dna from a broad range of sample types cells, blood, tissues, and more. The most versatile benchtop automation in the lab for consistent extraction and purification of dna, rna, protein, and cells. Qiaquick gel extraction kit protocol using a microcentrifuge. Purification of total dna from animal saliva using the.
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